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Home | Seminars and Symposia | Past seminars/symposia: Friday, February 10, 2006

Design, construction, and performance of the most efficient E. coli bacterium


Friedrich Srienc
Department of Chemical Engineering and Materials Science
BioTechnology Institute

Friday, February 10, 2006
11:15 am

402 Walter Library

We have applied inverse metabolic engineering based on elementary mode analysis to maximize the biomass yield of E. coli. Elementary mode analysis was first used to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only six genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain. Thus, inverse metabolic engineering is not just a theoretical concept but can be practically realized.