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Home | Seminars and Symposia | Past seminars/symposia: Friday, September 22, 2006

Mechanisms of budding yeast mitosis: Integrated computational and experimental analysis


David Odde
Biomedical Engineering

Friday, September 22, 2006
12:00 Lunch
12:15 Seminar

402 Walter Library

Download slides (pdf 2.4 MB) The proper segregation of the replicated genome, known as mitosis, is one of the most fundamental biological processes. To accomplish segregation, eukaryotic cells use a dynamic microtubule-based structure known as the mitotic spindle. We have developed computational models for the dynamics of mitotic spindle microtubules and the associated movements of chromosomes. These studies were used to model and predict the behavior of fluorescence associated with specific spindle components. We found that the microtubles are subject to spatial regulation of their dynamics at the nanoscale, so that one complete set of chromosomes is located in half of the spindle, and that the other complete set is located in the opposite half-spindle. In addition, we found that the spindle uses tension between sister kinetochores as an error correction mechanism to maintain the proper separation between sister kinetochores. Our analysis establishes a computational framework that models not only the underlying dynamics of the system, but also the observation of those dynamics at the nanoscale via digital fluorescence microscopy, an approach we call “model-convolution.”